The killing effect of suicide gene therapy system of HSV-TK/GCV and IL-12 for cervix cancer

Objective To study the killing effect of suicide gene therapy system of HSV-TK/GCV and IL-12 for hela cells of cervix cancer in vitro.Methods Hela cells was transfected with HSV-TK and IL-12 gene by lipofection and used in vitro study.We'll observed the killing effect of GCV for hela cells which transfected with HSV-TK and IL-12 gene and the bystander effect.Results The hela cells which transfected with HSV-TK and IL-12 gene was obviously changed in appearance after 24 hours which added GCV,but the normal control cells growthed prosperity.The survival rate of the hela cells which transfected with HSV-TK and IL-12 gene was fall-off with the plus of the GCV.The killing effect of GCV for the hela cells transfected with HSV-TK and IL-12 gene was more strengthener than that for the normal cells.As well as obviously bystander effect of suicide gene therapy system was observed.Conclusion HSV-TK/GCV and IL-12 gene was transfected into hela cells by lipofection and was expressed stability.There's obviously killing effect of HSV-TK/GCV and IL-12 for the hela cells with GCV.