Effect of Human Ribosomal Protein L6 on Multidrug Resistance in K562/A02 Cells

Objective To investigate the effect of ribosomal protein L6 (RPL6) gene on the resistance of leukemia cell to antitumor drugs.Methods RPL6 cDNA was obtained by RT-PCR ,Both sense and antisense cDNA recombinants of RPL6-encoding gene were constructed with pcDNA3.1(+) expression vector ,then the sense RPL6 cDNA recombinant was transfected into K562 cells while the antisense one into K562/A02 cells by liposomal reagent.The chemosensitivity of cells was detected by MTT assay.Results Internal control RT-PCR showed higher expression of RPL6 in K562/A02 than in K562;Sense-transfected K562 cells were 3.25 times more resistant to adriamycin than control cells did,whereas resistant to adriamycin of antisense-transfected K562/A02 cells decreased by 62% than that of control cells;Conclusion RPL6 gene plays an important role in the development of drug resistance in K562/A02 cells.