Effects of Bio-organic Fertilizer on Nicotine Accumulation of Flue-cured Tobacco

The nicotine contents of tobacco plants were determined to investigate effects of bio-organic fertilizer on nicotine accumulation flue-cured tobacco when different amount of bio-organic fertilizer plus other chemical fertilizers was applied as the basic manure under the same N application amount.The results show that the nicotine accumulation amount does not increase with increase of N absorbing rate.The N absorbing velocity reach the peak at 50d after transplant.The nicotine accumulation amount is slower within 50d after transplant,increases sharply over 50d after transplant,reaches the maximum at 80d after transplant and the same situation occurs in the treatments with applying the bio-organic fertilizer except for the nicotine accumulation amount is higher than CK before the squaring stage.The nicotine contents of root,stem and leaf of the treatments are affected by top pruning.The nicotine content of tobacco root is the highest and the nicotine contents of stem and leaf is almost the same before top pruning.The nicotine content of tobacco leaf is greatly higher than that of root and the nicotine content of stem is the lowest after top pruning.The total nicotine accumulation amount of the treatments with applying bio-organic fertilizer is higher than that of the treatment with applying pure chemical fertilizer.