Study on the optimum condition of Aspergillus niger solid state fermentation to produce pectinase

Pectinase was produced by Aspergillus niger through solid fermentation.By-products of agriculture processing products were applied as major materials.The results showed that the suitable culture medium within the scope of experiment was composed of bran and soybean meal(ratio of 8 to 2),3.5% ammonium sulfate.Initial pH of medium was nature.The optimized fermentation condition was that 15 g medium was packed in 250 mL flask,ratio of water and medium material was 1∶1.5,the inoculum size was 106 spores per gram mediun.Activity of pectinase produced on optimized condition reached 2262 U/g per gram medium.