Effect of amplified autologous NK and T cells infusion from peripheral blood lymphocyte on tumor immunotherapy

Objective: To study the change of blood lymphocyte phenotype after amplified autologous NK and T cells infusion from peripheral blood lymphocyte in 22 patients with cancer.Methods: Mixture(named NKTm) of NK and T lymphocytes cells amplified from peripheral blood in malignant tumor patients was infused with 2 weeks interval.Before infusion and 2 weeks after infusion,the lymphocyte phenotype was detected by flow cytometry included CD3+、 CD3+CD4+、 CD3+CD8+、 CD3-CD16-CD56+、 CD3-CD16+CD56-、 CD3-CD16+CD56+、 CD3+CD16-CD56+、 CD3+CD16+CD56-、 CD3+CD16+CD56+.Results: Two weeks after infusion,the phenotype of CD3+、 CD3+CD8+、 CD3+CD4+ cells did not significantly change,while the NK like cells(marked with CD16+/CD56+) statistically incresesd(P0.01).The results did not changed after multiple doses.No side-effects were found.Conclusion: Amplified autologous NK and T Cells infusion from peripheral blood lymphocyte may increase the antitumor immune without any side-effects.