Enhanced biodegradation of reactive dye by immobilized white rot fungi Phanerochaete chrysosporium

In order to enhance enzyme production and degradability of dyestuff,white rot fungus Phanerochaete chrysosporium immobilized with polyurethane foam carriers was investigated in agitated Erlenmeyer flasks.Results showed that the decolorization of 95%was achieved in the immobilized system only after incubating Phanerochaete chrysosporium for 5 d,which was higher than 15%in the free suspended culture for 7 d.The maximum activity of MnP was 963.61 U/L in the immobilized culture,while it was 269.52 U/L in the suspended culture.Moreover,the consumption of carbon and nitrogen substrates in the immobilized culture was much faster than that in the free suspended culture,and the production of H 2 O2 in immobilized culture was higher than that in the free suspended culture.It can be concluded that the enhanced biodecolorization of reactive dyes in immobilized culture of Phanerochaete chrysosporium is due to the increased activity of MnP and H2 O2 concentration caused by fast consumption of carbon and nitrogen substrates in the culture system.