Helicobacter Pylori Infection and Expression of π Glutathione S-transferase in Intestinal Metaplasia

Objective:To study the relationship between the virulence of H.pylori and human carcinogen detoxification system in the stage of intestinal metaplasia(IM).Methods:About 219 cases were examined in the study,including 30 cases with normal gastric mucosa,171 cases with IM and 18 cases with gastric cancer.The expression of GST-π were detected by SP method.The types of IM were classified into Ⅰ,Ⅱ,Ⅲ by high-iron diamine /alcian blue pH2.5/periodic acid Schiff(HID-ABpH 2.5-PAS)method:H.pylori infection was confirmed or excluded by hematoxylin-eosin(HE) staining,H.pylori-DNA PCR and enzyme-linked immunosorbent assay(ELISA).Eighty cases with H.pylori infection were received the treatment of H.pylori eradication.Results:The GST-π expression was not found in normal mucosa,the positive rate of GST-π in gastric cancer was 44.4%.The positive rate of GST-π expression in IM without H.pylori infection was higher than that of IM with H.pyloriinfection(P0.05).The positive rate of GST-π expression in H.pylori eradicated group was significantly higher after H.pylori:eradication than before(P0.01).The risk of gastric cancer is increased if there was low or no expression of GST-π in H.pylori-infected cases.Conclusion:The carcinogen detoxification role of GSTπ and the virulence of H.pylori might interact each other in the stage of IM,which is the precancerous condition of gastric cancer.