2015The Korean Journal of Pesticide ScienceOpen access

Sensitivity to Sterol Biosynthesis Inhibitors of Colletotrichum gloeosporioides Isolated from Persimmon in 2013 in Sangju, Gyeongsangbukdo

Tae Heon Lim, Dong Woon Lee, Oh Gyeong Kwon, Sang‐Sub Han, Byeongjin Cha, In Kyu Song

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Abstract

Colletotrichum gloeosporioides탄저병균은 우리나라 감 재배지 주요 병의 하나이다. 2013년 상주 지역 감(Diosprosi kaki) 과원에서 C. gloeosporioides에 의하여 감염된 신초, 과일 및 잎으로부터 병원균을 분리하여 prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, tebuconazole+tolyfluanid 등 총 5종의 살균제에 대한 반응을 조사하였다. 분리된 67 균주 모두 공시 약제의 포장적용 농도에서 균사생육이 완전히 억제되었다. Tebuconazole의 $EC_{50}$ 값의 범위는 $0.02-1.04{\mu}g/ml$ 이었으며 평균 $0.31{\mu}g/ml$ 이었다. Pochloraz manganese complex의 $EC_{50}$ 값의 범위는 $0.02{\sim}0.23{\mu}g/ml$ 이었으며 평균 $EC_{50}$ 값은 $0.078{\mu}g/ml$ 이었다. Fluquinconazole+Prochloraz(FP)에 대한 $EC_{50}$ 값을 조사한 결과, 평균 $0.029{\mu}g/ml$ 이었다. $EC_{50}$ 값에 기초한 약제간 상관계수 r은 tebuconazole과 prochloraz manganese complex간에는 0.42이었으며, prochoraz manganese complex와 FP 합제 간에는 0.44, tebuconazole과 FP 합제 간은 0.27이었다. Colletotrichum gloeosporioides is one of the most serious pathogens of persimmon in Korea. In 2013, 67 isolates of C. gloeosporioides were isolated from infected fruits, leaf and twigs of persimmon (Diosprosi kaki) at Sangju, Gyeongsangbukdo and fungal responses against five fungicides (prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, and tebuconazole+tolyfluanid) were evaluated by their growth on the fungicide-medium. All isolates were inhibited mycelium growth on the medium with each recommended application concentration of flied. $EC_{50}$ ( ${\mu}g/ml$ ) of tebuconazole was from 0.02 to 1.04 and average was 0.31. $EC_{50}$ ( ${\mu}g/ml$ ) of prochloraz manganese complex was 0.02~0.23 average was 0.078. Average $EC_{50}$ values ( ${\mu}g/ml$ ) of Fluquinconazole+Prochloraz (FP) was 0.029. On the basis $EC_{50}$ ( ${\mu}g/ml$ ), the correlation coefficient (r) between tebuconazole and prochloraz manganese complex, prochloraz manganese complex and FP, tebuconazole and FP were 0.42, 0.44 and 0.27, respectively.

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Colletotrichum gloeosporioides탄저병균은 우리나라 감 재배지 주요 병의 하나이다. 2013년 상주 지역 감(Diosprosi kaki) 과원에서 C. gloeosporioides에 의하여 감염된 신초, 과일 및 잎으로부터 병원균을 분리하여 prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, tebuconazole+tolyfluanid 등 총 5종의 살균제에 대한 반응을 조사하였다. 분리된 67 균주 모두 공시 약제의 포장적용 농도에서 균사생육이 완전히 억제되었다. Tebuconazole의 $EC_{50}$ 값의 범위는 $0.02-1.04{\mu}g/ml$ 이었으며 평균 $0.31{\mu}g/ml$ 이었다. Pochloraz manganese complex의 $EC_{50}$ 값의 범위는 $0.02{\sim}0.23{\mu}g/ml$ 이었으며 평균 $EC_{50}$ 값은 $0.078{\mu}g/ml$ 이었다. Fluquinconazole+Prochloraz(FP)에 대한 $EC_{50}$ 값을 조사한 결과, 평균 $0.029{\mu}g/ml$ 이었다. $EC_{50}$ 값에 기초한 약제간 상관계수 r은 tebuconazole과 prochloraz manganese complex간에는 0.42이었으며, prochoraz manganese complex와 FP 합제 간에는 0.44, tebuconazole과 FP 합제 간은 0.27이었다. Colletotrichum gloeosporioides is one of the most serious pathogens of persimmon in Korea. In 2013, 67 isolates of C. gloeosporioides were isolated from infected fruits, leaf and twigs of persimmon (Diosprosi kaki) at Sangju, Gyeongsangbukdo and fungal responses against five fungicides (prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, and tebuconazole+tolyfluanid) were evaluated by their growth on the fungicide-medium. All isolates were inhibited mycelium growth on the medium with each recommended application concentration of flied. $EC_{50}$ ( ${\mu}g/ml$ ) of tebuconazole was from 0.02 to 1.04 and average was 0.31. $EC_{50}$ ( ${\mu}g/ml$ ) of prochloraz manganese complex was 0.02~0.23 average was 0.078. Average $EC_{50}$ values ( ${\mu}g/ml$ ) of Fluquinconazole+Prochloraz (FP) was 0.029. On the basis $EC_{50}$ ( ${\mu}g/ml$ ), the correlation coefficient (r) between tebuconazole and prochloraz manganese complex, prochloraz manganese complex and FP, tebuconazole and FP were 0.42, 0.44 and 0.27, respectively.

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Available abstract

Colletotrichum gloeosporioides탄저병균은 우리나라 감 재배지 주요 병의 하나이다. 2013년 상주 지역 감(Diosprosi kaki) 과원에서 C. gloeosporioides에 의하여 감염된 신초, 과일 및 잎으로부터 병원균을 분리하여 prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, tebuconazole+tolyfluanid 등 총 5종의 살균제에 대한 반응을 조사하였다. 분리된 67 균주 모두 공시 약제의 포장적용 농도에서 균사생육이 완전히 억제되었다. Tebuconazole의 $EC_{50}$ 값의 범위는 $0.02-1.04{\mu}g/ml$ 이었으며 평균 $0.31{\mu}g/ml$ 이었다. Pochloraz manganese complex의 $EC_{50}$ 값의 범위는 $0.02{\sim}0.23{\mu}g/ml$ 이었으며 평균 $EC_{50}$ 값은 $0.078{\mu}g/ml$ 이었다. Fluquinconazole+Prochloraz(FP)에 대한 $EC_{50}$ 값을 조사한 결과, 평균 $0.029{\mu}g/ml$ 이었다. $EC_{50}$ 값에 기초한 약제간 상관계수 r은 tebuconazole과 prochloraz manganese complex간에는 0.42이었으며, prochoraz manganese complex와 FP 합제 간에는 0.44, tebuconazole과 FP 합제 간은 0.27이었다. Colletotrichum gloeosporioides is one of the most serious pathogens of persimmon in Korea. In 2013, 67 isolates of C. gloeosporioides were isolated from infected fruits, leaf and twigs of persimmon (Diosprosi kaki) at Sangju, Gyeongsangbukdo and fungal responses against five fungicides (prochloraz manganese complex, tebuconazole, mancozeb+myclobutanil, fluquinconazole+prochloraz, and tebuconazole+tolyfluanid) were evaluated by their growth on the fungicide-medium. All isolates were inhibited mycelium growth on the medium with each recommended application concentration of flied. $EC_{50}$ ( ${\mu}g/ml$ ) of tebuconazole was from 0.02 to 1.04 and average was 0.31. $EC_{50}$ ( ${\mu}g/ml$ ) of prochloraz manganese complex was 0.02~0.23 average was 0.078. Average $EC_{50}$ values ( ${\mu}g/ml$ ) of Fluquinconazole+Prochloraz (FP) was 0.029. On the basis $EC_{50}$ ( ${\mu}g/ml$ ), the correlation coefficient (r) between tebuconazole and prochloraz manganese complex, prochloraz manganese complex and FP, tebuconazole and FP were 0.42, 0.44 and 0.27, respectively.

Key concepts: Tebuconazole, Myclobutanil, Fungicide, Horticulture, Manganese, Chemistry, Botany, Biology

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Sensitivity to Sterol Biosynthesis Inhibitors of Colletotrichum gloeosporioides Isolated from Persimmon in 2013 in Sangju, Gyeongsangbukdo — Research Paper | ScholarLens