The Expression of Neuropeptide Ghrelin in Amygdala- kindled Seizure in Rats

Objective To observe the expression level of neronpeptide chrelin in blood serum and brain tissue in amygdala- kindled rat after seizure and to explore its causes and significance. Methods Sixty- four healthy male SD rats were randomly divided into blank control group( n = 8),sham- operated group( n = 8)and amygdala- kindled group( n = 48). No treatment was performed in the blank control group. Implantation of electrodes was performed in the sham- operated group. Implantation of electrodes and eletrophotoluminescence were performed in the amygdala- kindled group to induce amygdala- kindled seizure modles. Rats in the amygdala- kindled group was divided into 6 sub- groups( n = 8) at different times after kindling( 3,6,12,24,48,and 72 h). Enzyme linked immunosorbent assay,immunohistochemistry staining and real- time quantitative polymerase chain reaction were employed to detect the altered Ghrelin in blood and brain tissue of amygdala- kindled rat after seizure. Results Compared with sham- operated group,expression level of acylated Ghrelin decreased significantly( P 0. 05) in serum and brain tissue of amgydala- kindled rats after seizure. The expression of acylated Ghrelin were consistent with trend at each time point in serum and hypothalamus. No significant difference on expression of acylated Ghrelinw was found between the sham- operated group and blank control group( P 0. 05). Conclusion The expression level of acylated Ghrelin in serum could reflect level of acylated Ghrelin in hypothalamus. It could be one of Clinical indicators on diagnose and therapy assessment of epilepsy.