Experimental Study on Microleakage of Three Different Fissure Sealants

Objective: To provide experimental evidence by study on microleakage of three different fissure sealants.Methods: Forty human maxillary premolars,caries-free for orthodontic reasons were collected and randomly divided into five groups.Fissures were respectively sealed with conventional pit and fissure sealant(Helioseal F),glass ionomer cement(GC Fuji VII),GC Fuji VII plus saliva contamination,wetbond sealant(Embrace WetBond),Embrace WetBond plus saliva contamination.Six teeth in each group were stained with 50% silver nitrate for 2 hours,then were sectioned for evaluation of microleakage.Two other teeth in each group were used to observe the marginal adaptability of fissure sealants and interface condition by scanning electronic microscope.Results: Without saliva contamination,there were significantly differences concerning the microleakage results among three different sealants.Helioseal F was fully penetrated into the bottom of the fissure by SEM,together with the enamel completely.While with saliva contamination,GC Fuji VII and Embrace WetBond had a poor sealing ability,the interface between sealants and enamel appeared significant micro-gap.Conclusion: The pit and fissure sealant Helioseal F had a excellent marginal adaptation.The new glass ionomer sealant GC Fuji VII and wet-bond sealant Embrace WetBond only needed a technology with lower difficulty,and could achieve a acceptable marginal adaptation,their sealing abilities needed further clinical trials.