2005•Zhonghua jianyan yixue zazhiRequires access
Integron-mediated multidrug resistance in clinical multi-reisistant Pseudomonas aeruginosa
Zhang Xiu-zhe
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Abstract
Objective To investigate the action of integron-mediated multidrug resistance in clinical multi-resistant Pseudomonas aeruginosa.Methods The strains of multi-resistant Pseudomonas aeruginosa were selected with K-B susceptibility method. The three-dimensional method was taken to differentiate the various beta-lactamases. The integron was determined by PCR with integron-specific primer. DNA sequencing was also used to analyze resistance-related gene in the integron in multi-resistant Pseudomonas aeruginosa.Results The positive rate of integron detection in multi-resistant Pseudomonas aeruginosa was 42.4%.Three kinds of different length integron were found .They were 750bp,1 000bp and 2 500bp and aadB,aadA2,aadB,aac6-II and PSE-1 were the resistance-related gene cassette in the integrons.Conclusions Integron took part in mutiple resistance in multi-resistant Pseudomonas aeruginosa.We should pay more attention to integron-mediated horizontal transference of mulidurg resistance to lessen the occurrence of multi-resistant strains.