[Effects of resveratrol on morphology and oxidative stress of brain tissues in aging mice].

OBJECTIVE: To investigate the protective effects of resveratrol on brain tissues in aging mice induced by D-galactose. METHODS: 54 Kunming male mice aged 16 weeks were randomly divided into control group, aging group, intervention group. The control group was prepared by subcutaneous injection of normal saline and the other two groups were prepared by subcutaneous injection of D-galactose(200 mg/kg BW). The intervention group was fed with resveratrol (22.5 mg/kg BW)by oral gavage and the other two groups were fed with solution of 0.5% sodium carboxymethyl cellulose. All animals were sacrificed 16 weeks later, morphology of brain was observed, calculate the organ coefficients, and GSH-Px, SOD, CAT, MAO activity and MDA content were detected in the mice brain. RESULTS: Compared with the control group, the aging group lost normal morphological structure of nerve cells and the number of nerve cells was significantly decreased (P < 0.05), the organ coefficients were significantly decreased (P < 0.05), and GSH-Px, SOD, CAT activities were significantly decreased (P < 0.05), MAO activity and MDA content were significantly increased in brain of the aging group (P < 0.05). Compared with the aging group, the intervention group brain maintain normal morphological structure of nerve cells and the number of nerve cells was significantly increased (P < 0.05), the organ coefficients were significantly increased (P < 0.05), and GSH-Px, SOD, CAT activities were significantly increased (P < 0.05), MAO activity and MDA content were significantly decreased in brain of the intervention group (P < 0.05). CONCLUSION: Resveratrol can maintain normal morphological structure of nerve cells of aging mice, decrease oxidative stress responses, and has protective effects on brain tissues in aging mice induced by D-galactose.