Association between the position-654G/A and-1429T/A single nucleotide polymorphisms(SNP) existing in 5′-regulatory region of β_2-adrenoceptor(β_2-AR) gene and risk of essential hypertension in Uighur of Xinjiang

Objective:To detect single nucleotide polymorphisms(SNPs) existing in 5′-regulatory region of β2-adrenoceptor(β2-AR) gene and to investigate association of the identified SNPs with essential hypertension in Uighur of Xinjiang.Methods:Single nucleotide polymorphisms(SNPs)in the position-654G/A and-1429T/A of β2-AR gene were tested with MALDI-TOF-MS and Genotypes of the SNP were typed by MassARRAY iPLEXTMmethod.Results:Two SNPs were identified at position-654 with G→A substitution and-1429 with T→A substitution.The frequency of genotype of the two SNPs complied well with the Hardy-Weinberg equilibrium in normal group Distribution of genotype AA,GA,GG of the SNPs at locus-654 in hypertension group was no significantly different from that in normal group(P0.05),No significant difference was observed in distribution of geno-types of the SNPs at locus-1429 between the two groups(P0.05).Conclusion:These results indicate that the SNPs at locus-654 and-1429 of β2-AR gene were the SNPs position but not linked to hypertension.