Rapid detection of Escherichia coli O157: H7 using immunocaptured PCR

PCR tubes were coated with the monoclonal antibody against hemorrhagic Escherichia coli O157 to capture the suspected bacteria in samples.PCR was carried out to detect the captured bacteria.Results showed that the sensitivity of this method was up to 102CFU / mL.Compared with direct PCR and the ELISA,the sensitivity of this method was increased by 1 000 times.The PCR test in the other 10 non-target strains showed negative results,which revealed that the immunocaptured PCR method had high specificity.This method combined immunology detection with molecular biology detection.The immunological enrichment and amplification of PCR happened in the same PCR tube.This method not only improved the detection sensitivity and accuracy,but also shortened the detection time.Also,it reduced the detection cost and pathogenic loss.This method is a new rapid detection method which is suitable for on-site test.