Bio-detection and Identification of Biochemical Substances in Pinus massoniana Leaves

Pinus massoniana is a specific landrace species and long-term production practices of forestry indicate that the continuous cropping of pure P. massoniana forests can result in decreased P. massoniana yields. This study adopted Supercritical Fluid Extraction to extract biochemical substances from P. massoniana leaves and germination test to biologically detect and identify these substances. The results of the bio-detection indicated that the substances extracted only with CO2 and the substances extracted with the mixture of CO2 and ethanol showed different effects on the germination of P. massoniana seeds. As a whole, the substances extracted only with only CO2 appeared to inhibit the germination rate and radicle length and fresh and dry weight of P, massoniana seeds to a varying extent but to increase the germination potential and hypocotyl length of the seeds and the substances extracted with the mixture of CO2 and ethanol appeared to inhibit the germination rate and hypocotyl length and fresh and dry weights of P. massoniana seeds but to increase the germination potential and radicle length of the seeds. The results of the i- dentification revealed that of the substances extracted only with CO2,the major ones were benzaldehyde and cis-2,6-Dimethyl-2,6-octadiene and of the substances extracted with the mixture of CO2 and ethanol, the major ones were β-linalool and phenethyl alcohol.