Preparation, Purification and Certification of Antibody to Corticosteroids

Three immunogens and coating-antigens were prepared by conjugating derived dexamethasone, betamethasone and flumethasone with bovine serum albumin, ovalbumin respectively. Each immunogen was applied to immunize two New Zealand rabbits to obtain anti-serums. After five immunization, the antiserum was collcted from the blood, and purified by salt fractio combined with DEAE. The indirect ELISA was adopted to determine the efficiency of the purified antiserum. Among all the antiserum samples, the better efficiency of the antiserum could be as high as 345600, 614400 and 307200 respectively, which had sufficient affinity to meet the requirements of antigen-antibody reaction kinetics.