Expression and Identification of Capsid Protein of Foot-and-mouth Disease Virus Type O in Insect Cells

The capsid protein precursor P12 A and protease 3C coding P12A3C gene of FMDV type O were amplified from the plasmid p MD19-P1A3C,then the gene P12A3C were inserted into the baculovirus transfer vector p Fast Bac Dual to construct recombinant transfer vector p FastBac Dual-P12A C,in which the P12A3C gene were under the control of PH promoter.The recombinant baculovirus plasmids were transformed into Escherichia coli DH10 BacTMto construct the recombinant baculovirus bacmid Bacmid-P12A3C,then transfected into Sf9 cells,the recombinant baculovirus was harvested when obvious cytopathic effect was observed.After amplied,the baculovirus were infected into the Sf9 cells.At last the expressed proteins were analyzed by the immunouorescent assay.The result indicated that the expressed protein were expressed in Sf9 cells,and displayed specificity to FMDV type O VP1 polyclonal antibody.From this study,the recombinant baculovirus including the virus capsid P1 gene and 3C protease gene coding regions of FMDV type O were successfully obtained,this provides a basis for the research of FMDV type O empty capsid assembly in vitro and empty capsid vaccine reaserch.