Optimization of ethanol extraction process of total flavonoids from Xinjiang jujube by response surface methodology

To optimize the ethanol extraction of total flavonoids from Xinjiang jujube,the effects of extraction temperature,ethanol concentration,liquid to solid ratio and their interactions on the extraction yield of jujube flavonoids were studied with Box-Behnken design.The predictive polynomial quadratic equations model was developed by response surface methodology software.The optimum extraction conditions were obtained as follows: extraction temperature 66℃,ethanol concentration 60%vol and liquid to solid ratio 10.0.Under the optimal conditions,the extraction rate of total flavonoids was 2.32mg/g,which agreed well with the predictive value,indicating the optimized process was feasible.