Large-scale cultivation of porcine hepatocyte spheroids

Objective To establish a method of cultivation large scale liver parenchyma and noparenchyma cells and to form the porcine hepatocyte spheroids. Methods We performed spheroid in 1 L magnetic culture vessels. Results We obtained approximately 2×10 10 ～5×10 10 hepatocyte spheroids one time and observe the hepatic special structure in hepatocyte spheroids in a electric microscope. They expressed three times more albumin secretion and twice the ammonium removal as compared with conventional monolayers. Conclusions The method of cultivating large scale liver parenchyma and noparenchyma cells of hepatocyte spheroids can provide enough hepatocyts one time for bioartificial liver support systems.