Isolation and Purification of Sclerotinia sclerotiorum Strains in Rapeseed and Their Pathogenicity Identification in Guizhou

Pathogenicity of S.sclerotiorumstrains in rapeseed from 10geographic origins in Guizhou was identified by strains inoculation on the rapeseed leaves in vitro during seedling stage.The results showed that 10strains were isolated and purified among the 58samples,among them,the pathogenicity of stain ID 7-3from Sinan County was the strongest,followed by the strain ID 1-3from Suiyang County,there was significant difference of pathogenicity between both strains,but the strain ID 10-3from Xifeng County had the weakest pathogenicity. Therefore,there was obvious pathogenicity among the S.sclerotiorumstrains in different geographic origins in Guizhou,both of the two strains with strong pathogenicity isolated and purified could be used as reference strains for S.sclerotiorum resistance identification in future studies.