Isolation and Enzyme Screening of Halophiles from Barkol Lake and Manasi Lake

Objective:To understand the diversity of halophilic archaea and bacteria diversity and the extracellular hydrolytic enzymes diversity of Barkol lake and Manasi lake. Methods: Water samples were collected and the phylogenetic diversity of halophilic bacteria associated with the water was investigated. Protease, easterase, amylase, lipase and cellulase from these strains were screened. Results: 51 strains of halophilic bacteria were isolated. On the basis of the homology and phylogenetic analysis of 16S rDNA, it was considered that the halophilic bacteria were identified as members of halophilic bacteria from the two lakes were related to the genera Planococcaceae, Bacillaceae, Staphylococcus, halomonadaceae, Salicolaceae, Pseudomonadacaeae and extremely halophilic archaea strains were identified as members of the generaHalobacteriaceae. 15 strains produce protease, 23 strain produce esterase, 8 strains produce amylase and no strain produce lipase and cellulase. Conclusion: It was indicated that there are abundant resource of halophilic microorganism and enzyme in Barkol lake and Manasi lake.