2008•Dongbei Nongye Daxue xuebaoRequires access

Analysis of RAPD marker between parents and hybrids in melon

Luan Feishi

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Abstract

The genetic differences between parent lines and hybrids were detected from the molecular level used by RAPD markers,11 different melons were amplified by 27 RAPD primers,total 44 alleles were detected,and 19 alleles had polymorphism,which was about 43.18%,revealing that 3-8 bands per primer could be amp-lified,with an average of 1.63.Based on the analysis of genetic distance by RAPD markers,hybrids were close to genetic distance to maternal lines than fathers;the genetic distance between parent lines was 0 to 0.39.Compared with TopMark and H16 as male parent lines,hybrids inherited more genetic character from TopMark than H16.The results were similar to the performance of melon in the open filed.Using RAPD markers to analyze the genetic diversity can help breeders on parents selection and marker-assisted selection on melon breeding.

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What this paper is about

The genetic differences between parent lines and hybrids were detected from the molecular level used by RAPD markers,11 different melons were amplified by 27 RAPD primers,total 44 alleles were detected,and 19 alleles had polymorphism,which was about 43.18%,revealing that 3-8 bands per primer could be amp-lified,with an average of 1.63.Based on the analysis of genetic distance by RAPD markers,hybrids were close to genetic distance to maternal lines than fathers;the genetic distance between parent lines was 0 to 0.39.Compared with TopMark and H16 as male parent lines,hybrids inherited more genetic character from TopMark than H16.The results were similar to the performance of melon in the open filed.Using RAPD markers to analyze the genetic diversity can help breeders on parents selection and marker-assisted selection on melon breeding.

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Available abstract

The genetic differences between parent lines and hybrids were detected from the molecular level used by RAPD markers,11 different melons were amplified by 27 RAPD primers,total 44 alleles were detected,and 19 alleles had polymorphism,which was about 43.18%,revealing that 3-8 bands per primer could be amp-lified,with an average of 1.63.Based on the analysis of genetic distance by RAPD markers,hybrids were close to genetic distance to maternal lines than fathers;the genetic distance between parent lines was 0 to 0.39.Compared with TopMark and H16 as male parent lines,hybrids inherited more genetic character from TopMark than H16.The results were similar to the performance of melon in the open filed.Using RAPD markers to analyze the genetic diversity can help breeders on parents selection and marker-assisted selection on melon breeding.

Key concepts: RAPD, Hybrid, Biology, Melon, Genetic diversity, Genetics, Allele, Genetic distance

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