Isolation of polyclonal anti-idiotypic antibodies to AIV by aldehydated erythrocyte
Niu Zhong-xiang
Abstract
Niu Zhong-xiang
Abstract
Polyclonal anti-idiotypic antibodies to AIV were prepared from rabbits vaccinated with purified chicken anti-AIV antibodies, and isolated by aldehydated chicken red blood cells to bind unspecific chicken antibodies and to absorb rabbit-anti-chicken antibodies. The acquired antibodies were capable of agglutinating chicken red blood cells and inhibiting AIV in hemagglutinating weekly, and competed original AIV in binding chicken anti-AIV antibodies.
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Polyclonal anti-idiotypic antibodies to AIV were prepared from rabbits vaccinated with purified chicken anti-AIV antibodies, and isolated by aldehydated chicken red blood cells to bind unspecific chicken antibodies and to absorb rabbit-anti-chicken antibodies. The acquired antibodies were capable of agglutinating chicken red blood cells and inhibiting AIV in hemagglutinating weekly, and competed original AIV in binding chicken anti-AIV antibodies.
Key concepts: Polyclonal antibodies, Antibody, Biology, Virology, Isolation (microbiology), Molecular biology, Microbiology, Immunology