Selection of Relative Gene during Secondary Hair Follicle Growth in Cashmere Goat

In order to select the genes which regulate the growth of secondary hair follicle in cashmere goat.This study choose cashmere goat and dairy goats to be the material.Because these 2goats get the similar genetic background,but significant differences traits of the secondary follicle.An Illumina Hiseq2000 platform was used to sequence the 32 skin samples from 4cashmere goat and 4dairy goat on March,June,September and December.The Sequencing obtained 38 Gdata,check out 20 780 goats genes.We identified 2 409 differentially expressed genes between cashmere goat and dairy goat in the same period,1 175 cashmere goat genes were up regulated compared with dairy goat,1 234 were down regulated.And there were a total of 550 differentially expressed genes between different growth periods in cashmere goat,302 cashmere goat genes were up regulated,248 were down regulated.GO functional annotations showed that the main differentially expressed genes appear in the cells and cell parts,organs parts,binding,biological regulation,cellular process.KEGG pathway analysis found that 42 differentially expressed genes appear in WNT,SHH,TGFβ,TNF,MAPK and Notchsignaling pathways which intimately associated with hair follicle growth.This study provided valuable information to verify secondary folliclegrowth related gene function,provided an important basis to reveal the secondary follicle growth mechanism and provided the theory to increase production and quality of cashmere.