Optimization of pretreatment for determination of soluble sugar in straw

In order to determine the optimal pretreatment condition of soluble sugar in corn stover and wheat straw,different factors were studied,Box-Behnken and RSM were used for the optimization of pretreatment and regression models were obtained.The results showed that the ratio of straw to water,water-bath temperature,extraction time and stored time had significant effects,P0.05,on the yield of soluble sugar in corn stover and wheat straw.The optimal pretreatment condition of soluble sugar in corn stover is that the ratio of straw to water is 0.31 g/mL,water-bath temperature is 94 ℃,extraction time is 63 min,then the yield of soluble sugar in corn stover reach 3.85%.the optimal pretreatment condition of soluble sugar in wheat straw is that the ratio of straw to water is 0.33 g/mL,water-bath temperature is 95 ℃,extraction time is 58 min,then the yield of soluble sugar of wheat straw reach 3.39%.