2003Journal of Xingjiang Normal UniversityRequires access

Effect of Melatonin on Oocyte Spontaneous Maturation of Mouse in Vitro

Zhu Changqing

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Abstract

Mouse oocytes were cultured in maturation medium, and the effects of MT on meiotic maturation of mouse oocyte were examined. The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.but not in DOs. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.

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What this paper is about

Mouse oocytes were cultured in maturation medium, and the effects of MT on meiotic maturation of mouse oocyte were examined. The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.but not in DOs. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.

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Available abstract

Mouse oocytes were cultured in maturation medium, and the effects of MT on meiotic maturation of mouse oocyte were examined. The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.but not in DOs. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.

Key concepts: Oocyte, Melatonin, Meiosis, In vitro maturation, In vitro, Andrology, Chemistry, Cell biology

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