Effect of Melatonin on Oocyte Spontaneous Maturation of Mouse in Vitro
Zhu Changqing
Abstract
Zhu Changqing
Abstract
Mouse oocytes were cultured in maturation medium, and the effects of MT on meiotic maturation of mouse oocyte were examined. The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.but not in DOs. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.
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Mouse oocytes were cultured in maturation medium, and the effects of MT on meiotic maturation of mouse oocyte were examined. The results show MT at all doses of 0. 1mg/ml、0. 02mg/ml、0. 004mg/ml or 0. 0008mg/ml inhibited the formation of PB1 in CEOs cultured in maturation medium and ha no effect on GVBD.but not in DOs. Conclusion: MT is able to affect mouse oocyte maturation and the regulation mechanisms may be related to cumulus cells.
Key concepts: Oocyte, Melatonin, Meiosis, In vitro maturation, In vitro, Andrology, Chemistry, Cell biology