Healing-promoting effects of recombinant human granulocyte-macrophage colony-stimulating factor gel on residual burn wounds

OBJECTIVE To investigate the effects of recombinant human granulocyte-macrophage colony-stimulating factor(rhGM-CSF) gel on residual burn wound healing.METHODS The study was a randomized,double-blind,placebo-controlled and self-controlled clinical trial.One hundred and twenty residual burn wounds were divided into group A(n=60,with treatment of rhGM-CSF gel) and group C(n=60,with treatment of placebo),the patients were treated for 21 days totally.Wound healing rate on 7th,14th day after treatment and wound healing time were observed.The wound tissue samples(n=6) at different time points were obtained for taking count of blood capillaries and fibroblasts through optical microscope and evaluation of expression change of proliferating cell nuclear antigen(PCNA).RESULTS The wound healing rate in group A was(62±13)%,(95±10)% respectively on 7th,14th day after treatment,which was obviously higher than that in group C [(46±11)%,(83±12)%,P0.01].The mean wound healing time was 12.6(95% confidence interval 11.9 to 13.3) days in group A,which was obviously shorter than that in group C [ 16.8(16.1 to 17.5) days,P0.01].On 7th,14th day after treatment,histopathology showed the number of blood capillaries which was(10.3±0.6),(14.5±0.9) and fibroblasts which was(143.1±10.3),(137.8±6.9) in group A was obviously improved as compared with group C(7.2±0.7),(10.4±0.8)(P0.01);(110.2±11.7),(126.4±7.7)(P0.01) respectively,the expression intensity of PCNA in wound tissue of group A was also significantly higher than that in group C.CONCLUSION rhGM-CSF gel can accelerate wound healing in patients with residual burn wounds.