Distribution and antibiotic resistance of bacterial pathogens isolated during the period from 2008 to 2010 in department of hematology

Objective To investigate the distribution and antibiotic resistance of bacterial pathogens isolated during the period from 2008 to 2010 in Department of Hematology of Socond Hospital of shanxi Medical University.MethodsClinical isolates were identified and subjected to antimicrobial susceptibility testing by means of a unified protocol.The results were analyzed according to CLSI breakpoints(2008,2009 and 2010).ResultsOf the 943 clinical isolates,gram positive and gram negative organisms accounted for 45.5%(429/943) and 54.5%(514/943),respectively.Coagulase negative Staphylococcus(CNS) was the most frequently isolated gram positive bacteria.The prevalence of methicillin-resistant strains was 17.6% in Staphylococcus aureus(MRSA),and 64.5% in coagulase-negative Staphylococcus(MRCNS).Vancomycin was the most active antimicrobial agent against gram positive cocci.No VISA or VRSA strain was identified.E.faecium strains were more resistant to most of the antibiotics tested than E.faecalis.No enterococcal strain was resistant to vancomycin or teicoplanin.Klebsiella strains were the most frequently isolated gram negative bacteria in Department of Hematology,accounting for 12.8%,followed by E.coli.Imipenem was the most active antibacterial agent tested against gram negative bacilli.About 13.2% and 11.6% of the A.baumannii strains were resistant to imipenem and meropenem,respectively in Department of Hematology.P.aeruginosa showed relatively lower resistance to ceftazidime,amikacin and ciprofloxacin.More than 20% of these P.aeruginosa strains were resistant to other antibiotics.ConclusionsPatients with hematological malignancies are susceptible to nosocomial infection,which should be paid more attention to in clinical and laboratory practice.Rational antimicrobial therapy is an effective way for control of antimicrobial resistance.