Inhibitory effect of the specific antisense oligodeoxynucleotide on Fas expression and T cell apoptosis induced by FasL

To investigate the inhibitory effect of the specific antisense oligodeoxynucleotide(ASODN) on Fas expression and T cell apoptosis induced by hepatocarcinoma cells,FasL expressed by hepatocarcinoma cell line HepG2.2.15 was detected by flow cytometry(FCM) and the function of the FasL-inducing T cell apoptosis was assayed by co-culture assay in vitro with HepG2.2.15 cells and Jurkat cells.The Jurkat cells were trasfected with Fas-ASODN through lipofectin.Meanwhile,the influence of Fas-ASODN on the Fas expression on T cells,Fas mRNA level and apoptosis was investigated through FCM,RT-PCR and co-cultures.It was found that HepG2.2.15 cells expressing the functional FasL could induce the apoptosis of Jurkat cells as demonstrated by co-culture assay,and the level of Fas mRNA,the rate of Fas expression and the apoptotic rate induced by hepatocarcinoma cell line were all decreased.As a conclusion,it is evident that hepatocarcinoma cells expressing FasL can induce the apoptosis of T cells,indicating that trasfection of Fas ASODN can partially convert the inhibitory effect induced by heptocarcinoma cells.