Cardioprotection effect of different concentration sevoflurance postconditioning on isolated rat heart with myocardial ischemia-reperfusion injury

Objective To investigate the cardioprotection effect of different concentration sevoflurance postconditioning on isolated rat heart with myocardial ischemia-reperfusion injury. Methods Ninety SD rats were randomly divided into 6 groups (n=15): group N was perfused for 150 min, while the other five groups were perfused for 30 min, ischemia for 30 min, and reperfused for 90 min. At the beginning of the reperfusion, these five groups were reperfused with K-H fluid containing 0% (group I), 1% (group S1), 2% (group S2), 3% (group S3), and 4% ( group S4) sevoflurane for 5 min, respectively. Left ventricular diastolic pressure (LVEDP), left ventricular developed pressure (LVDP) were recorded. After the reperfusion, acute infarct size, mitochondrial damage, and cytoplasm and mitochondrial levels of cytochrome C were evaluated. Results Compared with group N, LVEDP and cytoplasma cytochrome C levels increased and LVDP, mitochondria cytochrome C levels decreased in groups I, S1, S2, S3, and S4 (P0.05). Compared with groups I and S1, LVEDP and cytoplasma cytochrome C levels decreased and LVDP, mitochondria cytochrome C levels increased in groups S2, S3, and S4 (P0.05). After the reperfusion, infarct sizes were higher in groups S1 and I than in groups S2,S3,S4(P0.05). Conclusion Sevoflurance at the concentrations of 2%-4% protects against myocardial ischemia-reperfusion injury of isolated rat heart, which may be attributed to the decreased mitochondrial damage and cytochrome C release.