Genetic diversity of microsatellite DNA in six breeds of China sheep(goat)

In order to investigate the breed characteristics and genetic diversity of China sheep(goat),microsatellite markers were used in the present study for 6 breeds of china sheep(goat).Gene frequencies of 7 microsatellite loci of 123 Ujumuqin Sheep were examined by using the method of random sampling in typical colonies of the central area of the habitat,and the PCR amplifying and the PAGE electrophoresis.Relational data were cited to analysis the genetic differentiation of Ujumuqin sheep,Han sheep,Tan sheep,Hu sheep,Tong sheep and White Goat of Yangtze Triangle District.The results indicated there were genetic polymorphisms at seven microsatellite markers in these six sheep(goat) breeds.Analysis of the relations between six populations of sheep and goat based on standard genetic distance,DA genetic distance and fuzzy similarity relations,Hu sheep and Tong sheep clustered first,Ujumuqin sheep and Han sheep and then with Tan sheep,five sheep populations clusteredd with goat at last.