Investigation on enterotoxins and toxic shock syndrome toxin-1 (TSST-1) of mastitis Staphylococcus aureus

Enterotoxin genes and tst gene were investigated on 124 bovine clinical mastitis Staphylococcus aureus,213 bovine subclinical mastitis Staphylococcus aureus,and 12 goat mastitis Staphylococcus aureus by PCR.The results showed that:the positive rate of enterotoxin genes and tst gene was 27.42% in bovine clinical mastitis Staphylococcus aureus,1.41% in bovine subclinical mastitis Staphylococcus aureus,and 33.3% in goat mastitis Staphylococcus aureus.Enterotoxin A,enterotoxin C and TSST-1 were major toxins produced by bovine mastitis Staphylococcus aureus,but the goat mastitis Staphylococcus aureus produced enterotoxin C in a great measurement.All positive enterotoxin genes and tst gene were examined by ELISA or RPLA.The results showed that:one Staphylococcus aureus isolate did not produce enterotoxin A and four Staphylococcus aureus isolates did not produce TSST-1,but they were positive in PCR.