Cloning and sequencing of the gD gene of pseudorabies virus Min-A strain

A pair of primers were designed according to the sequences pulished by the GenBank in order to amplifiy gD gene of the pseudorabies virus Min-A strain.The gD gene were obtained by polymerase chain reaction(PCR), and then cloned into the pGEM-T Easy vector. The recombinant plasmid of pGTE-gD was identified by restriction enzyme analysis and sequencing ,Which proved completely its validity. Nucleotide sequencing revealed that this fragment contained an open reading frame of 1203 bp encoding a 400 aa protein. The gD geng is subcloned into pcDNA3.1-,an eukaryotic expressing vector.The constructed expressing plasmid pcDNA-gD will be used for future gene vaccination.