Effect of All-trans Retinoic Acid on the proliferation apoptosis in D407 human retinal pigment epithelial cells

【Objective】 To investigate the effect of All-trans Retinoic Acid(ATRA) on the proliferation and apoptosis in D407 human Retinal pigment epithelium cells induced apoptosis in vitro.【Methods】 D407 cells were cultured conventionally and divided into experimental group and blank group.The secondary cultured D407 cells were counted and then exchanged with the medium contained 10 μg/mL of ATRA as the tested groups,which were compared with the blank control group with normal medium.Then these cells were counted again after another 72 hours to analyze the inhibitory function by ATRA on both groups.The inhibition of the cultured D407 cells attachment by ATRA after 24 hours culture was studied.Light and electronic microscopy was used to observe morphological changes of D407 cells.The apoptotic rate of D407 cells in both testing group and control group cells were analyzed by flow cytometry.【Results】 The cell counts of the ATRA testing groups was prominently decreased than the blank control group.The most of the apoptotic D407 cells were detectable in early stage after 72 hours ATRA exposure.【Conclusion】 The ATRA can inhibit cultured D407 cells proliferation as well as attachment effectively and its inhibition mechanism may be related to cell apoptosis.