Effects of Exercise on Endoplasmic Reticulum Stress Induced the Apoptosis of Hippocampus Cells in the Alzheimer's disease Model Rats

Objective:To explore the effect and mechanism of exercise on endoplasmic reticulum stress induced the apoptosis of hippocampus cells in the Alzheimer's disease model rats.Methods:45 normal male SD rats were randomly divided into 3 groups with 15 rats in each group,the sham operation(S) group,the AD model(M) group,the exercise model(E) group.The AD model of rats were established by bilateral hippocampus stereotaxic injection of Aβ25-35.The apoptosis rate of hippocampus cells were detected with terminal-deoxynucleotidyl transferase mediated nick end labeling(TUNEL),and the protein expressions of GRP78,Caspase-12,Bcl-2 and Bax in hippocampus tissues were analyzed using Western blot.Results:The TUNEL-Positive neurons in hippocampus of rats in the group M significantly increased than that in the group S(P0.01).Western blot showed that the protein expressions of GRP78,Caspase-12,and Bax in the group M increased more significantly than that in the group S(P0.01) and the expressions of Bcl-2 was conversed.After 6 weeks swimming exercise,compared with the group M,the TUNEL-Positive neurons and the protein expressions of GRP78,Caspase-12,and Bax in the group E significantly decreased(P0.01) and the expressions of Bcl-2 was significantly increased(P0.01).Conclusion:The six-week swimming exercise reduced the apoptosis neurons in hippocampus.The mechanism may be that the swimming exercise depress the ER and down-regulated the expressions of GRP78,Caspase-12 and Bax and up-regulated the expressions of Bcl-2 and then promoted the survival of neurons.