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Cloning and Sequence Analysis of a cDNA Encoding ACC Oxidase from Carnation Flower

Catas Haikou

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Abstract

Total RNA was extracted from petals of carnation(Dianthus caryophyllus L.)and reverse transcribed to first strand of cDNA.A pair of primers were designed and synthesized according to the sequence reported.The very specific PCR product of the1-aminocyclopropane-1-carboxylic acid oxidase was obtained by using the first strand of cDNA as template.The product was ligased to pGEMR-T easy vector and sequenced.The sequencing data showed that the PCR product was1156bp which contained a915bp coding region,encoding304predicted amino acid residues.Comparison of the cDNA sequence from this experiment with that reported by Savin Kw indicated the homology was100%.It was deduced that the ACO1gene in the species of Dianthus caryophyllus L.was highly conservative.

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What this paper is about

Total RNA was extracted from petals of carnation(Dianthus caryophyllus L.)and reverse transcribed to first strand of cDNA.A pair of primers were designed and synthesized according to the sequence reported.The very specific PCR product of the1-aminocyclopropane-1-carboxylic acid oxidase was obtained by using the first strand of cDNA as template.The product was ligased to pGEMR-T easy vector and sequenced.The sequencing data showed that the PCR product was1156bp which contained a915bp coding region,encoding304predicted amino acid residues.Comparison of the cDNA sequence from this experiment with that reported by Savin Kw indicated the homology was100%.It was deduced that the ACO1gene in the species of Dianthus caryophyllus L.was highly conservative.

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Available abstract

Total RNA was extracted from petals of carnation(Dianthus caryophyllus L.)and reverse transcribed to first strand of cDNA.A pair of primers were designed and synthesized according to the sequence reported.The very specific PCR product of the1-aminocyclopropane-1-carboxylic acid oxidase was obtained by using the first strand of cDNA as template.The product was ligased to pGEMR-T easy vector and sequenced.The sequencing data showed that the PCR product was1156bp which contained a915bp coding region,encoding304predicted amino acid residues.Comparison of the cDNA sequence from this experiment with that reported by Savin Kw indicated the homology was100%.It was deduced that the ACO1gene in the species of Dianthus caryophyllus L.was highly conservative.

Key concepts: Carnation, Complementary DNA, Dianthus, Biology, Petal, cDNA library, Sequence analysis, Rapid amplification of cDNA ends

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Cloning and Sequence Analysis of a cDNA Encoding ACC Oxidase from Carnation Flower — Research Paper | ScholarLens