Research of Caveolin-1 changing after delayed cerebral vasospasm in rat model of subarachnoid hemorrhage

Objective To observe the expression of Caveoion-1 in the basilal artery after delayed cerebral vasospasm(DCVS)in rat model of subarachnoid hemorrhage(SAH).Methods One hundred and fifty SD rats were randomly divided into blank group,saline group and SAH group.The SAH group and saline group were divided into three,five,seven and fourteen days after establishing model.SAH model was established by double injection of autologous arterial blood into the cisterna magana,test the changing of Caveoin-1 in the basal artery.Results(1)The situation of SAH model:in SAH group,all animals Bederson neurological severity scores ≥1,proving the success of model.(2)The situation of vasospasm:the lumen of basilar artery in the SAH groups lessened gradually and peaked on the 7th days,the thickness of vascular smooth muscle layer of basilar artery significantly increased(P 0.05).(3)The situation of Caveolin-1:the protein expression of Caveolin-1 by western blot test in the SAH groups decreased to the lowest point on the 7th days,significantly decreased compared with that in the saline groups and blank groups.Conclusion The protein expression of Caveoin-1 is down-regulated in basilal artery after DCVS in rat model of SAH,Caveoin-1 may be a potential inhibitor for DCVS and provide the direction for DCVS treatment.