The Construction of Dominant Negative Mutant Plasmid pCDNA4 - C - GFPand Its in vitro Expression

To investigate the construction of pCDNA4 - C - GFP and its in vitro expression. Methods The DNA plasmid pCDNA4 - C- GFP, which contains the C gene encoding core protein of HBV and green fluorescent protein gene, was constructed by gene technology. The plasmid pCDNA4 - C - GFP was transferred into the cell line HepG2. The in vitro expression of the plasmid was observed. Results Transcripts of the plasmid were detected by RT- PCR, and the GFP expession was detected by confocal. Conclusion The plasmid pCDNA4 - C- GFP can be used for its functional research.