Prilimilary Study on Antioxidant Activity of Ethanol Extracts from Ficus pumila L.Fruit
Lian Jun-rui
Abstract
Lian Jun-rui
Abstract
Fruit of Ficus pumila L.was extracted with various concentration(20%~95%) ethanol solution using ultrasonic at room temperature.The antioxidant activities of different extracts were evaluated by reducing power assay,DPPH assay,TEAC assay and FRAP assay,the total phenolics contents of the extracts were measured by Folin-Ciocalteu method.The results demonstrated that the antioxidant activities of different concentration ethanol extracts showed varying degrees of efficacy in four assays(reducing power,scavenging capacity on DPPH· and ABTS·+,the total antioxidant capacity).The concentration of ethanol solution significantly affected the antioxidant activities of extracts.Antioxidant activities of different ethanol extracts were descending in the following order: 80%,95%,60%,40%,20% ethanol,in which,80% ethanol extracts had the highest antioxidant activities,the absorbance value at 700 nm was 1.04,half scavenging efficiency concentration for DPPH· was 2.20 mg·mL-1,the value of TEAC and FRAP were 2.36,157.1,respectively.The results were in accordance with the highest total phenolics content(2.50%) of 80% ethanol extract.
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Fruit of Ficus pumila L.was extracted with various concentration(20%~95%) ethanol solution using ultrasonic at room temperature.The antioxidant activities of different extracts were evaluated by reducing power assay,DPPH assay,TEAC assay and FRAP assay,the total phenolics contents of the extracts were measured by Folin-Ciocalteu method.The results demonstrated that the antioxidant activities of different concentration ethanol extracts showed varying degrees of efficacy in four assays(reducing power,scavenging capacity on DPPH· and ABTS·+,the total antioxidant capacity).The concentration of ethanol solution significantly affected the antioxidant activities of extracts.Antioxidant activities of different ethanol extracts were descending in the following order: 80%,95%,60%,40%,20% ethanol,in which,80% ethanol extracts had the highest antioxidant activities,the absorbance value at 700 nm was 1.04,half scavenging efficiency concentration for DPPH· was 2.20 mg·mL-1,the value of TEAC and FRAP were 2.36,157.1,respectively.The results were in accordance with the highest total phenolics content(2.50%) of 80% ethanol extract.
Key concepts: DPPH, Antioxidant, ABTS, Chemistry, Ethanol, Food science, Absorbance, Oxygen radical absorbance capacity