Clinical Application of Early Rescue ICSI in Short-term in Vitro Fertilization Failure

Objective To evaluate the outcome of early intracytoplasmic sperm injection( ICSI) and late ICSI for in vitro fertilization and embryo transfer. Methods From June 2010 to June 2012,30 cases of 20hours after fertilization from Reproduction Center of Nanning Second People' s Hospital were classified into group A( late ICSI),67 cases of 6 hours after fertilization into group B( Early ICSI). The number of retrieved oocytes,fertilization rate,multi-PN rate,good quality embryo rate,frozen embryo rate and clinical pregnancy rate were compared between group A and group B. Results The results showed the number of retrieved oocytes( 12. 10 vs 10. 10,P 0. 05),fertilization rate( 84. 73% vs 79. 92%),frozen embryo rate( 24. 90%vs 24. 10%) of Group B and Group A were not statistically significant( χ2= 1. 754,0. 923,0. 173,P 0. 05),but the rates of multi-PN( 2. 53% vs 0. 00%),good quality embryo( 40. 72% vs 5. 64%) and clinical pregnancy( 40. 30% vs 10.00%) of group B were found higher than group A,with statistically significant differences( χ2= 2. 434,4. 560,8. 905,P 0. 05). Conclusion Early ICSI may be the best choice for in vitro fertilization failure rescue,which has similar clinical outcomes of IVF.