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Rapid Analysis of Paeoniae Radix Alba by Near Infrared Spectroscopy

Zhenzhong Wang

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Abstract

Objective:To develop a rapid NIR method for determination of paeoniflorin in paeony root. Method:HPLC was used as a reference method to determine the content of paeoniflorin.Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method.Result:the RMSECV of the model for paeoniflorin was 0.019 7,the correlation coefficients of the calibration models was 0.969 7,the RMSEP was 0.046 1.Conclusion:The NIR method was rapid,simple,and noninvasive and that is applied to determine the content of paeoniflorin in paeony root.It can offer reference to content determination of other Chinese herbs.

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What this paper is about

Objective:To develop a rapid NIR method for determination of paeoniflorin in paeony root. Method:HPLC was used as a reference method to determine the content of paeoniflorin.Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method.Result:the RMSECV of the model for paeoniflorin was 0.019 7,the correlation coefficients of the calibration models was 0.969 7,the RMSEP was 0.046 1.Conclusion:The NIR method was rapid,simple,and noninvasive and that is applied to determine the content of paeoniflorin in paeony root.It can offer reference to content determination of other Chinese herbs.

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Available abstract

Objective:To develop a rapid NIR method for determination of paeoniflorin in paeony root. Method:HPLC was used as a reference method to determine the content of paeoniflorin.Multivariate calibration model based on PLS algorithm was developed to correlate the spectra and the corresponding values determined by the reference method.Result:the RMSECV of the model for paeoniflorin was 0.019 7,the correlation coefficients of the calibration models was 0.969 7,the RMSEP was 0.046 1.Conclusion:The NIR method was rapid,simple,and noninvasive and that is applied to determine the content of paeoniflorin in paeony root.It can offer reference to content determination of other Chinese herbs.

Key concepts: Paeoniflorin, Radix (gastropod), Near-infrared spectroscopy, Chromatography, Calibration, Content determination, Chemistry, Analytical Chemistry (journal)

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