Effects of Qilan Granulates on expression of Bcl-2 and P53 in 4NQO-induced rat tongue carcinogenesis

Objective To examine the inhibitory effects of Chinese herb Qilan Granulates on expression of Bcl-2 and P53 in 4-nitro-quinoline 1-oxide(4NQO)-induced tongue carcinogenesis of rats.Methods A total of 150 SD rats were divided into five groups: carcinogenesis model group(group A),three administration subgroups(group B～D) and normal control group.Rats in group A,B,C,D were fed with 0.002% 4NQO dissolved in drinking water to induce tongue carcinogenesis of rats.Different concentration of herb Qilan Granulates was given to the rats in group B,C,D during oral carcinogenesis.Rats were sacrificed at 9,18,27 and 36 weeks respectively.The tongues of the rats were dissected for gross and histological observation,and two-step immunohistochemical method was also applied to detect the expression of Bcl-2 and P53 in the specimens.Results Compared with group A,the incidence of dysplasia and the expression of Bcl-2 and P53 were suppressed by Qilan Granulates(P0.05).Conclusion Administration of Qilan Granulates effectively suppressed 4NQO-induced tongue carcinogenesis.It may exert the chemopreventive effects by the regulation of Bcl-2 and P53.