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2006•Zhongguo shengwu huaxue yu fenzi shengwu xuebaoRequires access

Smad4 Participates in the TGF-β1-mediated ERK/MAPK Signaling Pathway

Xin He

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Abstract

To investigate the function of Smad4 in ERK/MAPK signaling pathway in human bronchial epithelial BEP2D cells. siRNAs were designed to specifically target Smad4 in BEP2D cells. siRNA transfection and western blots were performed to examine the phosphorylation levels of ERK and MEK. The results showed that Smad4 gene silencing leads to the phosphorylation level of MEK1 but not ERK decreased. After stimulation with TGF-β1, phosphorylation levels of both ERK and MEK are decreased and lower than the control cells. The results demonstrated that Smad4 is participating in the activation of the ERK/MAPK signaling by TGF-β1.

About this research paper

What this paper is about

To investigate the function of Smad4 in ERK/MAPK signaling pathway in human bronchial epithelial BEP2D cells. siRNAs were designed to specifically target Smad4 in BEP2D cells. siRNA transfection and western blots were performed to examine the phosphorylation levels of ERK and MEK. The results showed that Smad4 gene silencing leads to the phosphorylation level of MEK1 but not ERK decreased. After stimulation with TGF-β1, phosphorylation levels of both ERK and MEK are decreased and lower than the control cells. The results demonstrated that Smad4 is participating in the activation of the ERK/MAPK signaling by TGF-β1.

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Available abstract

To investigate the function of Smad4 in ERK/MAPK signaling pathway in human bronchial epithelial BEP2D cells. siRNAs were designed to specifically target Smad4 in BEP2D cells. siRNA transfection and western blots were performed to examine the phosphorylation levels of ERK and MEK. The results showed that Smad4 gene silencing leads to the phosphorylation level of MEK1 but not ERK decreased. After stimulation with TGF-β1, phosphorylation levels of both ERK and MEK are decreased and lower than the control cells. The results demonstrated that Smad4 is participating in the activation of the ERK/MAPK signaling by TGF-β1.

Key concepts: MAPK/ERK pathway, Phosphorylation, Gene silencing, Cell biology, Transfection, Signal transduction, Small interfering RNA, Stimulation

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