The effect of cocultured MPP+ induced PC12 cells and astrocytes on the expression of synaptophysin and GAP-43 in neural stem cells of Wistar rat

Objective To study the effect of cocultured l-methyl-4 phenyl pyridinium(MPP +) induced PC 12cells and astrocytes on the expression of synaptophysin and GAP-43 in neural stem cells of Wistar rat.Methods PC 12cells and MPP + were cocultured at different time(0h,24h,48h,72h,96h)as control,then the apoptosis rates of PC 12 cells induced by MPP+ were measured by Flowcytometer.MPP+ and PC12 cells and astrocytes were incubated for 48 hours,then all cells-conditioned medium was collected and used to culture neural stem cells(NSCs).The expression of synaptophysin(SYN) and growth-associated protein-43(GAP-43) in NSCs of all groups were detected by immunofluorescence.Results The apoptosis rate of PC12 cells induced by MPP + was the highest at 48h culture time,but lower at other culture hours(P0.05).The expression levels of synaptophysin(A value = 34.09 ± 2.69) and GAP-43(A value = 49.36 ± 5.98)in NSCs induced by astrocytes-conditioned medium cocultured for 48h were significandy upregulated compared to non astrocytes(P0.05).Conclusion Coculture of astrocytes and MPP + and PC 12 cells promotes the expression levels of synaptophysin and GAP-43 in NSCs.