Effect of Cadmium Chloride Administration by Oral Intubation on the Activities of Enzymes and Distribution of Cadmium in Organs of Female Rats

Thirty adult female SD rats were allocated into five groups of six each.Four groups(from Ⅰ to Ⅳ) were intubated orally with 1 mL of solutions contained Cd 0,0.2,1.0,2.0 mg as cadmium chloride,respectively once daily for 10 d.Group Ⅴ were given the same dose Cd as group Ⅳ for 30 d.The results showed that the activities of ALT and ARG in the plasma of rats exposed to cadmium significantly increased(P0.05) compared with that of control,but there was no regular dose-effect relationship of ALT activity;The activity of ALT in hepatic homogenate decreased significantly(P0.05,or P0.01);The activity of GSH-Px in whole blood significantly decreased(P0.05),and so did in their hepatic homogenate.The concentrations of cadmium accumulated in heart,liver and kidney obviously increased with dosing,and there was the positive correlation between cadmium levels accumulated and the dose given(r= 0.938 4, 0.968 7, 0.937 9,respectively);The concentration of cadmium in heart,liver and kidney of group Ⅴ was higher than group Ⅳ,and the increased rate of cadmium accumulated in the kidney was faster than that in the liver.It was concluded that cadmium chloride administration may retard the growth of rats.Cadmium may significantly inhibit the activity of GSH-Px,reduce the ability of anti-oxidation in tissues and induce injury of parenchymal cells such as liver.ARG activity in plasma may be considered as a comonitory index of cadmium in acute cadmium exposed animals.It should be taken care when the activity of ALT in the plasma is used as a characteristic to monitor parenchymal organs injury induced by cadmium.