Protective Effect of Panax japonicus Ethanol Extract on the Inflammation of RAW264. 7 Cells Induced by LPS

Objective: To observe the protective effect of Panax japonicus ethanol extract on the inflammation of mice mononuclear macrophage RAW264. 7 cells induced by lipopolysaccharide( LPS). Method: The RAW264. 7 cells were treated with different concentration of P. japonicus ethanol extract,cell viability was determined with MTT method; the nitric oxide( NO) release of RAW264. 7 cells stimulated by LPS and treated with LPS and P. japonicus ethanol extract was detected with Griess. ELISA were used to detect the expression of Tumor necrosis factor-α( TNF-α),interleukin 1β( IL-1β); nitric oxide synthase mRNA( iNOS mRNA) and neclear factor-κB( NF-κB) was tested by PCR and Western bolt respectively. Result: The safe range of ethanol extract of P. japonicus is less than 100 mg·L- 1; Concentration of LPS was 1 mg·L- 1; Compared with the LPS model group,P. japonicus ethanol extract 0. 1,1,10,40 mg ·L- 1could inhibit the of NO effectively,the inhibition rates were 31. 2%,41%,46. 1%,55. 2%,and inhibit the release of TNF-α,IL-1β,with significant difference( P 0. 05 or P 0. 01); P. japonicus ethanol extract 10,40 mg ·L- 1could down-regulate the expression of iNOS mRNA and inhibit NF-κB nuclear translocation. Conclusion: P. japonicus ethanol extract has protective effects on RAW264. 7 cells stimulated by LPS,the mechanism of P. japonicus ethanol extract may be related to it's regulation on NF-κB signal pathway,thereby inhibiting the release of NO and reducing the expression of TNF-α,IL-1β and iNOS.