Microsatellite Enrichment by Magnetic Beads in Guinea Pig (Cavia porcellus)

Objective To isolate microsatellite loci from guinea pig(Cavia porcellus) genome.Methods The 200 ～ 1000 bp DNA fractions of guniea pig containing microsatellite sequences were captured by hybridization with the oligonucleotide probes attached to streptavadin coated magnetic beads.The enriched DNA were ligated into pMD-18T and then transformed into E.coli DH5α competent cells.The method of PCR was used to screen positive clones from the libraries.And microsatellite primers were disigned based on the sequences from the positive clones.Results In total 240 positive clones were identified from about 2000 transformants in the libraries screened by PCR.Finally,we got 98 sequences and successfully designed 17 pairs of microsatellite primers for guniea pig.Conclusions The results showed that this method is very efficient to isolate microsatellite markers and the markers are useful tools for further studies on guinea pig genetics.