The Research in Hippocampus Neuron Damage after Global Brain Ischemia in the C57black/6 Mice

The objective of this paper is to provide experimental basis for a new model of global cerebral ischemia,the model is induced by bilateral common carotid arteries occlusion(BCCAo) in C57Black/6 mice.Several methods are used.Transient global ischemia is induced by bilateral common carotid arteries occlusion for 10 or 30 minutes,the mice are killed after 28 days,then brains are made to paraffin sections used for hematoxylin and eosin staining,and the mice are also killed in the 1st day,3rd day,5th day,7th day after operation,brains are made to frozen sections stained with TUNEL and PI staining.Several results are obtained.Firstly,in the global cerebral ischemia,the neuronal cells in CA1 region of 10min and 30min groups are both damaged.Secondly,30 minute group is serious damaged.Finally,the pyramid neuronal cells in CA1 region of 30min group are killed by apoptosis and necrosis.In the C57Black/6 mice,the global cerebral ischemia for 30 min is more easily induced necrosis,and the model is better than other models simulated clinical cerebral ischemia.