Hind III polymorphism in lipoprotein lipase gene: association with hyperlipidemia and coronary heart disease

AIM: To explored the relationship between lipoprotein lipase(LPL) gene polymorphic marker Hind Ⅲ at intron 8 and the severity of coronary heart disease.METHODS: Polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP) was used to detect the LPL gene Hind Ⅲ(polymorphism) in 208 patients.The severity of coronary artery disease(CAD) was determined by the number of significantly stenosed major coronary arteries at angiography(50% luminal obstruction).(RESULTS): We found a significant association between Hind Ⅲ polymorphism and the number of significantly diseased vessels(P0.05).There was also a close relationship between Hind Ⅲ polymorphism and levels of triglyceride and high density lipoprotein cholesterol((P0.01) respectively).(CONCLUSION): The Hind Ⅲ polymorphism in lipoprotein lipase gene is significantly associated with CAD severity,independent of changes of circulating lipid and lipoprotein levels,which may be involved in atherogenesis.