2006•Unpublished venueRequires access
Effect of ANP and Endothelin-1 on Hypertrophy and Proliferation of Cultured Cardiac Myocytes and Cardiac Fibroblasts
You Hong-wen
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Abstract
ObjectiveTo investigate the effect of ANP and endothelin-1(ET-1) on hypertrophy and proliferation of cultured cardiac myocytes and Cardiac Fibroblasts. Methods Cardiomyocytes and cardiac fibroblasts were isolated by trypsin digestion method. DNA and protein synthesis were measured by 3 H-deoxythy-midine( 3 H-TdR) and 3 H-Leucine( 3 H-Leu) incorporation. Protein content was measured by Bradford method. ANP mRNA expression of cardiomyocyte was assessed by RT-PCR. Results ET-1 signifcantly promoted while ANP reduced DNA synthesis, protein synthesis and pp ET-1 mRNA expression in a dose dependent manner in cultured cardiac myocytes. Analogously, ET-1 enhanced 3 H-TdR and 3 H-Leu incorporation rate in cultured cardiac fibroblasts, which was attenuated by ANP. Conclusion ANP inhibits cardiomyocytes hypertrophy and on cardiac fibroblasts proliferation induced by ET-1.